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| A1R+, A1+
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| High-speed, high-resolution imaging to capture intracellular biological processes
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The A1 series is Nikon's powerful new fully-automated confocal imaging system, capable of capturing high-quality confocal images of cells and molecular events at high speed and enhanced sensitivity. The A1R with hybrid scanner is ideal for advanced research methods using photo activation imaging. The A1 series has been designed with groundbreaking new optical and electronic technology innovations to provide unprecedented system quality and flexibility.
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| A1R's hybrid scanner for ultrahigh-speed imaging and photo activation
Ultrahigh-speed imaging at 420 fps
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The A1R incorporates a resonant scanner with a resonance frequency of 7.8 kHz and allows high-speed imaging at 420 fps (512 x 32 pixels). Moreover, the field of view of the scanned area is approximately five times larger than that of the non-resonant scanner. The Nikon original optical clock generation method realizes high image quality even at the highest speed. The fiber-optic communication data transfer system can transfer data at a maximum of four giga bps.
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| High-speed photo activation imaging
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Because a non-resonant scanner and a resonant scanner are incorporated in one unit, photo activation and fluorescence imaging can be conducted simultaneously without a separate laser unit for photo activation. With a resonant scanner that can capture images at high speed, acquisition of rapid changes after photo activation is possible.
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| What is a hybrid scanner?
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This mechanism allows flexible switching or simultaneous use of two galvano scanners (resonant and non-resonant) with high-speed hyper selector.
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| Enhanced spectral imaging |
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Fast acquisition of a 32-channel spectral image
Fast spectral imaging at 24 fps (512 x 32 pixels) is possible.
Real-time spectral unmixing
Fast fluorescence unmixing during image acquisition is possible in less than a second.
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| Enhanced spectral imaging |
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Accurate spectral unmixing, which has a high reputation among users of the previous model, the C1si, provides maximum performance in the separation of closely overlapping
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Actin of HeLa cell expressing H2B-YFP was stained with Phalloidin-Alexa488.
Spectral image in the 500-692 nm range captured with 488 nm laser excitation
Left: Spectral image, Right: Unmixed image (green: Alexa488, red: YFP)
Specimen courtesy of: Dr. Yoshihiro Yoneda and Dr. Takuya Saiwaki, Faculty of Medicine, Osaka Universityfluorescence spectra and the elimination of autofluorescence.
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| Filter-less intensity adjustment is possible with V-filtering function |
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Desired spectral ranges that match the spectrum of the fluorescence probe in use can be selected from 32 channels and combined to perform the filtering function. By specifying the most appropriate wavelength range, image acquisition with the optimal intensity of each probe is possible in FRET and colocalization. Up to four wavelength ranges can be simultaneously selected. The sensitivity of each range can be individually adjusted, which supports applications using various probe combinations.
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| Increased light detection efficiency realizes high image quality |
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Nikon has developed an original confocal microscopy VAAS (Virtual Adaptable Aperture System, option) that can eliminate flare while retaining image brightness. Because of the deconvolution of the light that passes through the pinhole and the light that does not pass through the pinhole, acquisition of brighter images with less flare is possible. Different sectionings (slice thicknesses) can also be simulated after image acquisition.
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| VAAS pinhole unit for bright, clear images |
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Dichroic mirrors in the scanning head employ the low-angle incidence method realizing a 30% increase in fluorescence efficiency. This allows brighter images and reduces laser exposure intensity, minimizing damage to cells.
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| NIS-Elements C?dedicated software with increased flexibility and ease of use |
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For diverse confocal microscope applications, Nikon provides easy-to-use software based on Nikon's leading imaging software NIS-Elements.
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| C2+
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| An essential microscopy laboratory instrument |
The C2+ confocal microscope system is part of a new generation of Nikon confocal instruments designed to be essential laboratory microscopy tools. Built on a reputation of incredible stability and operational simplicity coupled with superior optical technologies and high-speed image acquisition of up to 100 fps*, the C2+ is the perfect tool for a new microscope, or as a new accessory to an existing Nikon imaging system.
*With 8x zoom or larger
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| Large field-of-view imaging and three-dimensional reconstruction
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Confocal image acquisition, using high-numerical aperture and high-magnification objectives, together with XY stage control and advanced image stitching with Nikon NIS-Elements software, enables high-resolution images of large areas of a specimen to be produced. In addition, the microscope's high-precision Z-axis control allows assemblage of Z stack images for three-dimensional image reconstruction.
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| Image Quality
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Nikon's unprecedented optics and a time-proven, highly efficient optical design provide the brightest and sharpest images, at the longest working distances.
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| High-efficiency scanning heads and detectors
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With the convenient, small scan head size, the C2+ can be used with various types of Nikon microscope. The C2+ employs high precision mirrors and optically superior circular pinholes, and separates the detectors to isolate sources of heat and noise, enabling low-noise, high-contrast and high-quality confocal imaging. The newly
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| High-performance optics
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CFI Apochromat λS Series
These high-numerical aperture (NA) objectives are ideal for confocal imaging with correction of chromatic aberrations over a wide wavelength range from ultraviolet. In particular, the LWD 40xWI lens corrects up to infrared. Transmission is increased through the use of Nikon's exclusive Nano Crystal Coat technology.
- CFI Apochromat 40xWI λS, NA1.25 (left)
- CFI Apochromat LWD 40xWI λS, NA1.15 (middle)
- CFI Apochromat 60x Oil λS, NA1.4 (right)
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CFI Apochromat TIRF Series
These objectives boast an unprecedented NA of 1.49 (using a standard coverslip and immersion oil), the highest resolution among Nikon objectives. The temperature correction ring corrects image quality affected by temperature change in the range of 23°C to 37°C.
- CFI Apochromat TIRF 60x oil, NA1.49 (left)
- CFI Apochromat TIRF 100x oil, NA1.49 (right)
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| High-definition diascopic DIC images |
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The C2+ can acquire simultaneous three-channel fluorescence or simultaneous three-channel and diascopic DIC observation. High-quality DIC images and fluorescence images can be superimposed to aid in morphological analysis.
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| High functionality |
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High-performance imaging software NIS-Elements offers a variety of image processing and analysis functions. It also enables data extraction from acquired images. In addition, NIS-Elements allows for intuitive operation of Nikon microscopes and other third-party peripheral devices, such as EMCCD cameras and filter wheels, to broaden the range of experiments possible.
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| Multimode capability
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Various imaging methods, such as confocal, widefield, TIRF, photoactivation, as well as processing, analysis and presentation of acquired images, are available in one software package. Users can easily learn how to control different imaging systems with a common interface and workflow.
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| Unmixing
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Numerous functions for analysis and unmixing of acquired spectrums are provided, while spectral profiles of general dyes and fluorescent proteins are preprogrammed.
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| Flexibility
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The C2+ can be coupled with upright, inverted, physiological, and macro imaging microscopes and has options for combinations with various high-quality research experiment systems. All can be controlled with NIS-Elements software.
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| TIRF/Photoactivation-C2+ Multimode imaging system
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Optional TIRF laser illumination module and a photoactivation module can be integrated to enable both imaging of single molecules with an extremely high S/N ratio, and imaging of the fluorescence characteristic changes of photoactivated and photo-convertible fluorescent protein
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| AZ-C1
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| High-definition macro to micro confocal imaging
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The AZ-C1 enables high-definition confocal imaging during macro observation as well as micro observation.
Sharp wide field of view images with unprecedentedly high S/N ratios allow for imaging of whole-mount specimens such as embryos and large tissue slices that are commonly used in developmental and systems biology studies.
Moreover, the AZ-C1 offers a combination of low and high magnification objective lenses and a scanning zoom function, enabling continuous imaging from macro to micro with a single microscope.
New macro in vivo imaging capabilities allow for the capture of confocal images that were previously not possible with traditional stereoscopic microscopes.
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Comparison of same specimen regions captured by the AZ-C1 and an epi-fluorescence microscope The AZ-C1 eliminates out-of-focus light and flare to deliver highly resolved confocal fluorescence images and optical sections.
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| One-shot–whole specimen–macro confocal imaging
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High NA objectives for macro observation enable fast, high-resolution, single-image capture of a wide specimen area. Because the objectives cover a field of view larger than 1 cm, imaging of embryos during late stages of development and the dynamics of cell populations in whole organs are possible.
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The AZ-C1 can capture wide-field, optical sections at high resolution in a single scan. With a conventional confocal microscope, image stitching is necessary because the field of view that can be captured in a single scan is small.
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| Continuous imaging from low magnification to high magnification
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With five different objective lenses, optical zoom and confocal scan zoom, the AZ-C1 makes imaging possible from very low magnification to high magnification. Macro imaging, such as whole-section imaging, and micro imaging, including imaging of a single cell, can be done using a single microscope.
High magnification imaging offers clear and sharp images of single cells.
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| Deep imaging of whole specimens
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The AZ-C1 allows imaging deep into the specimen–difficult to achieve with conventional confocal microscopes. The AZ-C1 efficiently captures fluorescence signals from deep within a specimen in macro and in-vivo imaging.
Nerve cells (red) 2 mm beneath the surface of the embryo can be imaged clearly.
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| Confocal laser microscope system C1si/C1si-Ready/C1plus
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The Nikon C1 series offers the optimum confocal system to meet both your research and your budgetary needs.
C1plus: Standard model boasts high resolution, high sensitivity, and high contrast. Suited for single laboratories or large research groups.
C1si-Ready: Upgrade to C1si is possible by adding a spectral detector.
C1si: Spectral confocal system featuring a 32-ch multianode spectral detector. A spectral bandwidth of 320 nm can be captured in a single scan.
[Note] Upgrade to macro confocal microscope system AZ-C1 through combination with AZ100 microscope.
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Product image does not include the laser safety stage cover. The laser safety stage cover should be attached during actual use.
The diascopic detector is under development. Sample images captured with the prototype only.
AZ-C1 is a combination of the AZ100 microscope and the C1 series confocal laser microscope system. The AZ100M and A1 series are not compatible. . .
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